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What is the difference in permeabilization between the detergent-based and the methanol-based Perm buffers?

April 26, 2017Buffers difference perm permeabilization

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What is the difference in permeabilization between the detergent-based and the methanol-based Perm buffers?

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The detergent-based BD Perm/Wash buffers generally leave the secondary/tertiary structure of proteins intact, while methanol-based Perm buffers disrupt structural integrity. This disruption helps for Stat proteins that are dimerized, but may not be necessary for a protein that is not bound to anything else via its phospho-epitope.