What is the difference between the RampUP and RampUP Plus kits?
Both kits provide materials needed to amplify any intact or degraded RNA sample and generate sense-strand copies of the original RNA. RampUP Plus is unique in that it provides additional reagents needed to synthesize a poly(A) tail on the 3’ end of the amplified product. Therefore, the choice of kit is determined by whether or not a poly(A) tail is required for downstream analysis. If the downstream analysis utilizes random primers for reverse transcription, a poly(A) tail is not required and the RampUP kit will be sufficient. If, however, the application utilizes a dT primer for reverse transcription, the RampUP Plus kit is needed to generate a poly(A) tail.
