What is the difference between the pFIV-H1 Vector and the pFIV-H1/U6 Vector?
SBI offers two vectors for the cloning and expression of siRNA. The pFIV-H1 vector is an approach that utilizes a single siRNA sequence with a fold-back stem-loop structure (also known as a hairpin) that is expressed from a single promoter (H1). The sequence is then converted to a double-stranded siRNA after intracellular processing cleaves the loop. The pFIV-H1/U6-Puro vector is an approach in which the sense and anti-sense strands are transcribed separately from two independent promoters (H1 and U6) and form the siRNA duplex. A more detailed description of each system is available at www.systembio.
