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What is the difference between the H1 and the U6 promoters?

April 26, 2017H1 u6

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What is the difference between the H1 and the U6 promoters?

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The BLOCK-iT™ Inducible H1 and U6 Entry Vector Kits use either the Pol III dependent H1 or the U6 promoter, respectively. The H1 promoter is modified to contain two flanking tetracycline operator 2 (TetO2) sites within the H1 promoter. This allows the shRNA expressed from this promoter to be regulated in cells that express the tetracycline repressor (TR) protein. Both the H1 and the U6 are pol III type promoters; however, there may be some minor differences in their effectiveness depending on the cell line used.