What is the difference between the CMV and the CMV5 promoters?
A. The CMV5 is an enhanced version of the CMV promoter. The CMV5 promoter in our new AdenoVatorTM vectors was constructed by the insertion, downstream of the transcription start site of the human CMV IE promoter-enhancer, of the adenovirus tripartite leader (Ad-tpl) with the adenovirus major late enhancer bracketed by splice donor and acceptor sites. The Ad-tpl binds translation-initiating proteins much more efficiently than most messages. Viruses with CMV5 express about 6-10 folds more protein in 293 cells than viruses with CMV. Reference: Massie, B., F. Couture, et al. (1998). “Inducible overexpression of a toxic protein by an adenovirus vector with a tetracycline-regulatable expression cassette.” J Virol 72(3): 2289-96.
