What is the difference between R, HR and LR Bactosomes?
The activity of many cytochrome P450s can vary significantly with the amount of NADPH P450 reductase available. R and HR Bactosomes have high levels of NADPH P450 reductase and have, therefore, a high activity. This limits the linearity of substrate turnover with time. LR Bactosomes contain lower levels of NADPH P450 reductase than their R counterparts. These Bactosomes, while they have a lower Vmax for a given substrate, show enhanced linearity of substrate turnover with time . For a good illustration of this compare the product sheets for CYP3A4R and CYP3A4LR. The product that you choose will depend on your assay requirements.
