What is the difference between population doubling and passage number?
A population doubling is a two-fold increase in the total number of cells in a culture, and is most commonly referred to during the exponential, or “log”, phase of growth. The term passage number refers to the number of times that a cell population has been removed from the culture vessel and undergone a subculture (passage) process, in order to keep the cells at a sufficiently low density to stimulate further growth.
