What is the difference between in vivo and in vitro antibody production?
Monoclonal antibodies are produced by so-called hybridoma cells. Traditionally hybridoma cells were grown, or cultured, by injecting them to mice peritoneum. In in vivo production cells secrete antibodies to so-called ascites fluid and the antibodies can be purified from this fluid. In in vitro production cells are cultured in on a plate or in a flask or a flowpath using defined cell culture media. Q: What is the animal source for medixMAB antibodies? A: All medixMAB antibodies are derived from mice so if a secondary antibody is used rabbit anti-mouse or goat anti-mouse antibody should be used. However, it should be noted that after the hybridoma has been developed no animals are used for production. Q: What is hollow-fiber technology? A: In small-scale cell culture the cells are grown on a plate or in a flask. In order to maintain the culture, the medium has to be replaced frequently and usually this is done manually. Hollow-fiber bioreactors employ fibers with small pores that are pl
