What is the difference between GeneHub-GEPIS and the previous version of GEPIS?
top The GEPIS application was originally developed in 2004 to compute gene expression levels over different many normal and cancerous tissues using EST data. The application has been widely accessed and is an extremely valuable tool for expression analysis. The application was described by Zhang et al (Bioinformatics, 2004). Despite its success, the previous version of GEPIS has several limitations. First, its algorithm relies on the BLAST program to assign EST sequences to a given input mRNA sequence. We subsequently found that BLAST frequently link erroneous ESTs to input sequences due to high-percentage regional matches. As a result, a given EST could be matched to multiple genes, thus leading to false expression data. In addition, there were insufficient data for performing reliable analysis for mouse genes. Furthermore, the design of the algorithm did not allow easy access to the expression data through URL linking. Moreover, the detailed information about ESTs and their source li
