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What is the difference between DNA, RNA, and Methylation MLPA probes?

April 26, 2017difference dna Methylation mlpa probes RNA

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What is the difference between DNA, RNA, and Methylation MLPA probes?

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DNA and Methylation probes are used for the detection and quantification of chromosomal DNA sequences. The two probe oligonucleotides hybridise to immediately adjacent target DNA sequences. Methylation probes are similar to DNA probes, except that they contain a recognition site for the methylation-sensitive restriction endonuclease HhaI and are usually located within CpG islands. RNA probes are specific for cDNA sequences derived from mRNAs, and most probes will not generate any signal on contaminating DNA. This is because the hybridising parts of the two oligonucleotides of an RNA probe are located in adjacent exons, which are separated by an intron in chromosomal DNA.