What is the difference between automatic and manual Threshold Settings?
If manual is selected, the user sets the fluorescence value used for the threshold. Manual mode gives the user more flexibility to evaluate background. Automatic will always set threshold above background, however, it does not allow the user to choose the fluorescence value used for the threshold. Automatic mode calculates the threshold as the specified number of SDs above the background. Q: I made changes to the analysis table but the graph did not update to reflect changes. A: The Update Analysis button located at the bottom of the View Results screen must be selected to implement changes made to the Analysis Settings or Results Table.
