What is the difference between a TrueClone and a TrueORF?
TrueORFs are much more “engineered” than TrueClones. TrueORF inserts are just the coding sequence (Open Reading Frame aka ORF) in our pCMV6-Entry Precision Shuttle vector, designed to put C-terminal Myc-DDK tags on the expressed protein. TrueORFs do not contain any UTR sequences (these would interfere with making the fusion protein) but can be used for transient or stable overexpression in mammalian cells. The Open Reading Frame in any TrueORF can be easily shuttled to another destination vector adding various tags or functions.
