What is the difference between a normalized and non-normalized library?
Both libraries are “snapshots” of the gene expression for a particular organism, tissue and time. In a non-normalized library, each cell expresses thousands of copies of 10-20 highly abundant genes, hundreds of copies of hundreds (medium abundance) of genes, and only 1-50 copies of thousands of rare genes. When a cDNA library is normalized before sequencing, the normalization greatly decreases the multiple copies of abundant transcripts. Thus, normalization increases the efficiency of random sequencing dramatically, and is essential for rare gene discovery.
Related Questions
- There are several trial accesses available as on now. Please clarify whether ACM Digital Library & J-Gate will be available on regular basis or only just for few months?
- Why doesn the library provide access to all journal titles electronically?
- What is the difference between a normalized and non-normalized library?
