What is the difference between a “discrepancy” and a “mutation”?
A. ‘Discrepancy’ is a term borrowed from HIP that refers to differences between the actual clone sequence and the target sequence (that is, what the researchers were trying to clone in a specific cloning effort). Discrepancies can result when the clone recovered is an isoform or naturally occurring polymorphic form of the target sequence, or from PCR, replication, or other errors. Be sure to check the insert sequence to be sure that discrepancies that were deemed ‘acceptable’ to HIP researchers are also acceptable to you. ‘Mutations’ we define as changes that are known or expected to affect function. We curate an insert as containing a mutation when the insert sequence is a known mutant form (e.g. cloned from a mutant allele) or when the clone was engineered to have a mutation (e.g. in a site-specific mutagenesis effort). Please be aware that there may OR MAY NOT be experimental evidence to support the idea that a particular naturally occurring or engineered mutation results in a const
Related Questions
- Why does a mutation that deletes one or two DNA nucleotides changes gene function more drastically than a substitution of one nucleotide for another type?
- What type of mutation could result from a deletion of a single nucleotide from the DNA sequence?
- What is the difference between a "discrepancy" and a "mutation"?
