What is the detailed workflow of the DualChip GMO?
Following extraction of DNA from a putative GMO food or feed sample, three individual PCR reactions are performed with each sample, using the provided biotinylated primer cocktails for amplification of GMOs and plant-specific markers. Each PCR reaction is a multiplex PCR reaction for several targets. Afterward, the amplicons are combined, and hybridized to the microarray. The DualChip microarray component of each kit contains the detection sequences, or capture-probes, that are complementary in sequence to the amplified sequences. If hybridization between an amplicon and a microarray-linked capture-probe occurs, the biotinylated targets can then be detected with the Silverquant® detection technology. The Silverquant technology permits sensitive detection of the hybridized target in two steps: binding by an anti-biotin-antibody coupled to a gold-particle, followed by visualization of the silver precipitate formed on each hybridized target due to catalysis by the gold particle. The visua
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