What is the definition of “Tested” and “Untested” breeder pairs?
For some strains of mice carrying spontaneous mutations, heterozygous breeder pairs are identified by progeny testing rather than DNA typing. Progeny testing involves observing affected pups from breeder pairs of an unverified genotype. Mice proven to be heterozygous by breeding are sold as “tested” breeder pairs and may have produced one or more litters prior to distribution. “Untested” breeder pairs (noted by +/? x +/?) are of unknown genotype; these mice have not been bred prior to distribution. The purchase of more than one breeder pair is recommended to assure carriers of the spontaneous mutation of interest. Both untested and tested breeder pairs should produce progeny, the question is whether or not they produce affected pups. The best strategy for determining the genotype of a mouse with an unknown genotype (+/?) is to mate it to a known heterozygote (+/-) (e.g. mate an untested male or female with a tested male or female). Generally, if no homozygous mutant mice appear within
Related Questions
- Charter schools by definition are autonomous institutions. Why then do charter schools have to submit the Language Census and why do they have to submit it in conjunction with a particular LEA?
- There are lots of security term and definition pairs. What definitions does the stakeholder need to filter out before distributing a definition set to the RE team?
- What is the definition of "Tested" and "Untested" breeder pairs?
