What is the dead volume of Bio-Logic stopped-flow?
Dead volume of SFM-20 is 50 μl. Dead volume of SFM-300/400 is 150 μl. If you need to refill driving syringes (with same solution) during one experiment then you do not need to replace this volume. If you need to change concentration between shots, you do not need to refill the syringe as you can just change mixing ratio from the software. There is NO risk of dilution. The Bio-Logic mixer design also lets you save samples: for example if you need to try 10 concentrations then total priming volume would still be 150 μl with SFM-300.