What is the construction strategy of the physical map?
We are using a combination of approaches to provide contiguation of the zebrafish genome. Along with labs in Utrecht and Tüebingen, we have generated around 20 fold coverage of fingerprinted clones across the genome. These data are analysed and assembled at the Sanger, and released using Webfpc. The contigs generated are acting as a template for clone by clone sequencing of the genome. It is hoped these data will provide a useful substrate for positional cloning within the community. Contigs identified as of special interest to the community can be prioritised for walking. Please contact Sean Humphray for details.
We are using a combination of approaches to provide contiguation of the zebrafish genome. Along with labs in Utrecht and Tübingen, we have generated around 20 fold coverage of fingerprinted clones across the genome. These data are analysed and assembled at the Sanger. The contigs generated are acting as a template for clone by clone sequencing of the genome. It is hoped these data will provide a useful basis for positional cloning within the community. Contigs identified as of special interest to the community can be prioritised for walking. Please contact zfish-help for details.