What is the concentration of the primers and probes?
This will vary depending on what detection system you use. The classic TaqMan primers are usually at 900 nM and the probe at 200 nM. However, this can be re-optimized if needed. The concentrations will always be included on the provided setup sheet.The Amplifluor concentrations will always be the same: 5’ primer at 10 nM, 3’ primer at 100 nM, and the Amplifluor Uniprimer at 100 nM.
