What is the concentration of TCEP in the Immobilized TCEP Gel?
The concentration of TCEP is approximately 8 mM in the settled resin. However, take care when attempting to use this value to calculate the gel amount necessary for sample reduction because the reducing capacity of Immobilized TCEP is somewhat less than an equal concentration of free (non-immobilized) TCEP. Steric hindrance and diffusion to the immobilized TCEP affect the efficiency of interaction between sample molecules and the reductant.