What is the column “Spots Used” and where are the missing spots?
The number of “Spots Used” is the total number of spots (Log2 ratios) used for each gene to calculate the average Log2 ratio after removing data filtered by baseline, threshold and outlier definitions or flagged. The number of outliers removed are listed in the adjacent column and the “missing spots” are those filtered out by the baseline and threshold definitions or flagged. These filtered data points also appear on the “and A value for individual spots” report as missing data and are scored as “NA”.