Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

What is the chemical stability of the attached peptides?

April 26, 2017attached chemical peptides stability

0

Posted

What is the chemical stability of the attached peptides?

1 Answer

0

Posted

JPT Peptide Technologies offers two types of PepSpots™ peptide membranes: – PepSpots™: The connection between the peptide and the membrane is an ester bond and therefore labile towards strong bases (i.e. NaOH) and nucleophiles (i.e. NH2R). The peptides themselves are quite stable. However, strong acids may occasionally break acid labile amide bonds such as Asp-Pro. In addition, there are several amino acids such as Cys or Met which are sensitive towards oxidation. Finally, very strong bases (NaOH) may hydrolize peptide bonds as well. – PepSpots™ (PEG): The connection between the peptide and the membrane is an ether bond and therefore stabile towards strong bases (i.e. NaOH) and nucleophiles (i.e. NH2R). We suggest to use this membrane type if electroblotting experiments or application of alkaline phosphatase is planned.