What is the best way to wet the blot holder?
• Place a new, closed blot holder in the SNAP i.d. System, fill the wells with Milli-Q water and activate the vacuum until the wells empty. • Open the blot holder and submerge it into a shallow tray of Milli-Q water. Be sure to remove any excess water before loading a blot into the blot holder. The blot roller included with the SNAP i.d. System can used to remove the excess water. • Open the blot holder and apply Milli-Q water directly from a squirt bottle or with a pipette.