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What is the best way to propagate fresh isolates?

April 26, 2017best fresh isolates propagate

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What is the best way to propagate fresh isolates?

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The best cells to propagate fresh HHV-6A or HHV-6B isolates is to use PHA stimulated cord blood mononuclear cells or use human CD4+ T-cells which are the PCR negative PBMCs. One could also try the T-cell lines, i.e. HSB2, Supt., MOLT-3, J-Jhan, but success will be limited.