What is the best way to obtain DNA from the DCCT/EDIC relatives of the patients in whom we have found defective alleles?
A. Should you require assistance finding relatives, Helen Pan (hpan@rti.org) of the NIDDK Central Repository can help you identify the sample numbers you want to request. Q. I am attempting to assemble plasma and urine samples in a cohort of type-1 diabetics that have been followed a minimum of five years with annual assessments of estimated GFR (MDRD and/or cystatin C measurements are fine). My primary goal is to divide the samples into two groups: Group 1 – Will have an annual decrease in GFR less than or equal to 3.2% Group 2 – Will have an annual decrease in GFR greater than or equal to 3.2% Ideally, the two groups can be matched for sex, medication, BP control and albuminuria. Of these criteria for matching, the albuminuria is most important. Our initial studies will analyze the entry samples and use subsequent eGFR as a “look ahead” to define the natural history of the subjects’ DN A. If you consider just the DCCT dataset then, for all patient-visits where at least one aliquot (4
