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What is the best way to detect alkaline phosphatase activity?

April 26, 2017activity alkaline best detect phosphatase

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What is the best way to detect alkaline phosphatase activity?

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For easy detection of alkaline phosphatase activity, Invitrogen™ offers the ELF® 97 Endogenous Phosphatase Kit (SKU# E-6601). The kit includes the ELF® 97 phosphatase substrate that upon hydrolysis produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymatic activity. This fluorescent precipitate has several unique spectral characteristics, including an extremely large Stokes shift, 180 nm that makes it easily distinguishable from endogenous fluorescence.