What is the best to clean and wash small diameter particles?
The best way to clean and wash small particles is with tangential flow. Our recommended membrane is from Spectrum Laboratories, Inc. in Rancho Dominquez, CA. You can reach them at 310-885-4600 or at the web site, www.spectrapor.com. The membranes are sold under the trade name MiniKros. These are available in a range of sizes appropriate for volumes ranging from milliliters to liters. Pore sizes are readily available at 0.1 µM and these have been widely used by major diagnostics companies. Particles around 0.1 µM may be pelleted readily in floor model centrifuges at speeds of about 18K for times of 20 to 30 minutes. This is readily done with Sorval or Beckman Centrifuges. Volumes of 2 ml to 20 ml per tube may be readi8ly processed using round bottom centrifuge tubes designed to deal with the relatively high speed. Note that this speed is below that of an ultracentrifuge. During assay optimization it is preferable to use centrifugation in order to process numerous tubes in parallel. Once
