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What is the best methodology to use for DNase treatment?

April 26, 2017best dnase methodology treatment

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What is the best methodology to use for DNase treatment?

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We have always found a DNase treatment performed on a silca gel memebrane column to be the most effective for eliminating gDNA and void of possible cations that can be left behind and possibly inhibit enzymatic reactions with a post RNA recovery treatment. We recommend to add twice the amount of DNase units (27.3U) and double the incubation period (30 min) with an on-column treatment to insure all or most gDNA has been eliminated. If an “in tube” DNase treatment is used (such as the Turbo Kit by Ambion), be aware that downstream quantitation maybe artificially inflated.