What is the best method to use for extracting RNA from Cells?
The Qiagen RNeasy Mini (>105 cells) or Micro kits (<105 cells) generally work well for eukaryotic suspended and adherent cells, but most Silica column-Guanidium lysis reagents are fine. For samples that are suspect of being high in extracellular biomolecules such as polysaccharide, adipose, or similar, a silica column may perform poorly and Trizol should be used.