What is the best method for packing Toyopearl resin?
Toyopearl media is rated at a maximum pressure limit of 3 bar/ 45psi. This is the recommended pressure for achieving an optimally packed chromatography bed, however this may be limited by pressure constraints by instrumentation and equipment. Toyopearl resin should be packed in high salt buffer at a slurry concentration no greater than 60% to achieve maximum bed compression. Packing in salt results in a more consistent pressure drop across the column relative to packing in water at the same flow rate. After the bed height is stable under pressure, mark the bed height and lower the top adapter 0.2 – 0.5cm into the bed to prevent a void from developing during normal chromatography operation.
