What is the best electroporation system to use for bacterial electroporation?
We have a variety of electroporation systems, all of which are used for some bacterial work. In general, we recommend exponential decay for bacteria; however, square wave is used as well. If the application is simple (transformation with a plasmid under 20 Kb) and the bacteria is E. coli, then the ECM 399 may be used. You also have the option of electroporating a few mammalian cell lines with this unit. If more complex bacterial applications are needed, more mammalian cell lines are demanded, or specialty electrodes are to be used, the ECM 630 is the unit of choice to increase flexibility for all applications.