What is the anticipated yield of DNA per reaction? Will it be suitable for subsequent sequencing?
An average yield is about 20-50 ng and this is sufficient for next-generation sequencing. Note that recovered DNA is in single-stranded conformation and probably some optimization will be required for library creation for sequencing. Diagenode proposes the protocol for MeDIP-seq (please, look at the question/answer below), according to which the adaptors have to be ligated to DNA BEFORE MeDIP what will allow to use Illumina protocol.
