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What is the amount of sonicated DNA is needed to effectively visualize fragmentation on an agarose gel?

April 26, 2017agarose dna fragmentation gel needed sonicated Visualize

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What is the amount of sonicated DNA is needed to effectively visualize fragmentation on an agarose gel?

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The MagMeDIP kit manual recommends to use not more than 5µg of sonicated DNA per line. The migration of large quantities of DNA on agarose gel can lead to poor quality pictures, which do not reflect the real DNA fragmentation. We recommend optimizing and/or standardizing the required amounts based on your specific observations.