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What is the amount of RT-PCR product that can be achieved from a 1 µL PCR reaction?

April 26, 2017achieved PCR Product REACTION rt-pcr

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What is the amount of RT-PCR product that can be achieved from a 1 µL PCR reaction?

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Starting with 1000 transcripts the typical outcome of a 1 µL reaction on the AmpliGrid is 10 – 100 ng of RT-PCR product. Please have a look here for an example of the sensitivity achieved in a single cell multiplex rt-PCR reaction.