What is the advantage of working with a probe system?
The probe system is always specific; only detect the gene of interest. It is also possible to distinguish between similar sequences with small differences like SNPs or mutations. In general, probe assays need less optimisation than SYBR Green I assays. 3. Can I use a SensiMix SYBR Kit for a probe assay? This is not possible because the buffer for SensiMix SYBR kits and the buffer for probe kits are different, and have been optimised for their specific assays. 4. Why is the reaction volume 25µl or 50µl for this product? Most SensiMix reactions are 50µl with the exception of the SensiMix HRM Kit which is 25µl and SensiMix Capillary and SensiMix Capillary One-Step Kits which are 20µl. These are the volumes recommended by the machine manufacturers, and can be scaled up or down if desired (for example when using a 384 well plate). 5. Why do the SensiMix kits contain a hot-start Taq polymerase? Polymerase activity during the reaction set-up causes non-specific amplification including primer-
