What is the advantage of using VIC™ dye instead of JOE™ or HEX dye for a TaqMan® probe?
There are four main benefits: • VIC™ dye is two to three times brighter than JOE™ or HEX. Therefore, VIC™ dye provides for better sensitivity in a TaqMan® reaction. • VIC™ dye can be synthesized in all three synthesis scales. • The spectral emission of probes labeled with VIC™ dye is narrower than that of JOE™ dye, therefore, there is greater distinction between labeled probes in applications that require multiplexing. • The cost of synthesizing a VIC™ labeled probe is considerably less expensive than the synthesis with HEX and JOE. For more information on VIC™, please refer to User Bulletin 5, Multiplex PCR with TaqMan® VIC™ Probes.
