What is the advantage of using Normal Phase over Reversed Phased HPLC?
[top] Any advantage would be dependant on your sample and your separation goals. Generally, Normal Phase HPLC is better at separation of polar compounds then Reversed Phase. 6. Most acids and bases are not effective when the organic content of the mobile phase goes too high. Since I am interested to use the Cogent columns in ANP, what can I do to adequately “acidify” the mobile phase when organic is higher than 40%? [top] We have had some good success with methyl phosphonic acid. It remains soluble in acetonitrile content as high as 96%. This might help when some compounds are retaining in ANP but the peak shape needs to be tightened up a bit.
