What is the advantage of performing a 24-tissue RACE analysis if the tissue site(s) of expression of my gene is known?
By surveying more tissues where your gene is expressed, you markedly increase the probability of successfully completing the 5 end of your transcript of interest. Each cDNA synthesis yields a different proportion of full-length reverse transcripts because of differences in the intactness of the starting mRNA, in relative mRNA accumulation between tissues, and in the efficiency of cDNA synthesis between RNA populations. Additionally, the appearance of an identical PCR product in different tissues assures specificity of the RACE analysis.
Related Questions
- When performing gene expression analysis on the HumanHT-12 v4 chip, I get a message asking if I want to impute data for samples that have missing bead types. How do I impute data for this chip?
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