What is the actual volume of solutions that the IMAPlate 5RC96 will take up by using capillary force loading method?
A1: The geometric volume of each reaction chamber of the IMAPlate 5RC96 is 5µl. The actual volume of solution it takes up by using capillary force loading method is expected around 5.5 µl because the IMAPlate 5RC96 is designed to use this addition volume to obtain a better surface shape between liquid-air interface for the reader. The actual volume may have a slight change due to the surface tension of different liquids (as also seen in other liquid transfer device such as pipette). For example, the volume for taking up water is 5.6 µl with CV less than 2% (from 10 IMAPlate 5RC96). Therefore, it is recommended to perform a calibration when the IMAPlate 5RC96 is used as a liquid transfer device. For other applications such as micro-cuvette array or solid phase assay the difference between the geometric volume and the actual volume will have no influence on the result as long as the standards and samples are loaded by using the same method. Q2. How precise of the IMAPlate 5RC96 to take u
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