What is the ACTH dose for the stimulation test?
At 8 A.M., when cortisol secretion is at its normal diurnal peak, blood is drawn before and then 60 minutes following intravenous injection of a 0.25-mg bolus of ACTH. A nomogram that relates baseline to ACTH-stimulated serum concentrations of 17-OHP (17-hydroxyprogesterone) has been constructed which can be used to identify classical affected, nonclassical affected, and heterozygote carrier status.