What is SYBR Green®?
SYBR Green® is a fluorescent dye that binds to double stranded DNA with great specificity. It is widely used in molecular biology research to quantify and visualize double stranded DNA. SYBR Green® is a synthetic dye, which was introduced in the early 1990s by Molecular Probes®, Inc., a subsidiary of Invitrogen&trade Corporation. It is an asymmetrical cyanine dye, cyanines being a family of synthetic organic dyes that are used in photography, CD-R and DVD-R media, and in a variety of molecular biology research applications. Several features make SYBR Green® particularly useful as a nucleic acid stain. It is stable over a range of temperatures, which means that it can be used to monitor biochemical reactions that take place at high and low temperatures. It binds non-covalently to the surface of DNA, which is means that it will not interfere with the activity of most nucleases and DNA polymerases. It also binds to nucleic acids over a dynamic linear range of up to four orders of magni
