What is Sodium Azide and should I add it to the antiserum?
• Sodium Azide is a preservative that prevents bacteria from growing in the serum (bacteria can produce proteases that denature any proteins in the serum, including antibodies). We typically recommend adding azide unless the serum is going to be used directly in cell culture applications. Azide can be removed by using a dialysis membrane with a molecular weight cutoff between 12,000 and 14,000.