What is scale of synthesis?
Scale of synthesis refers to the amount of starting CPG (controlled-pore glass) support-bound monomer used to initiate the DNA synthesis, not the amount of final material synthesized. This is the same for all manufacturers of synthetic DNA using standard phorsphoramidite chemistry. When a 40 nmole scale synthesis is specified, approximately 40 nmoles of the first base is added to the DNA synthesizer. For an average 25-mer, at least 25% of this starting material will result in failure sequences, hence it is not possible to produce 40 nmoles of full-length product from a 40 nmole scale synthesis. The losses occur during synthesis, post-synthetic processing, transfer of material, and quality control. For oligos of standard length (<= 33 nucleotides) we guarantee a certain yield of DNA (OD260 applying to a 20mer) to be delivered within a range of +/- 20% according to the respective scale of synthesis and mode of purification.
Related Questions
- I received my oligonucleotide and the tube looks empty, or I have received several oligonucleotides, all of the same scale synthesis, and the amount of sample in each tube looks uneven. Why?
- How easy is a change from small scale synthesis on resin to SPOT synthesis on a membrane?
- What is a synthesis scale?
