What is nested PCR?
Nested polymerase chain reaction (PCR) is a modification of PCR intended to reduce the contamination in products due to the amplification of unexpected primer binding sites. Nested PCR utilizes two different sets of primers during a two-step amplification. The PCR reaction is run using the “outer primers”during a first cycle of amplification which is immediately followed by a second cycle of amplification carried out with the “inner primers”. This procedure increases the sensitivity of the assay by specifically amplifying only the desired product of the first reaction in a second reaction.This procedure significantly improves the specificity of amplification for the target cDNA.
