What is ment by “uncloned stable cell line” or “stable cell pool”?
After transfection, the cells are cultivated until only stably transfected cells are growing in the culture. For the “polyclonal” service, we check for transgene expression and deliver this cell pool of uncloned, stable transfected cells. For the “clonal” service, we subclone several 100 different cell clones from a stable cell pool, check for transgene expression and deliver clonal cell line(s) expressing the protein of interest.
