What is maximum amount of genomic DNA that can be used in a single IP?
Diagenode recommends 1 µg of DNA per IP assay, in our standard protocol from the MeDIP kit. The amount of DNA and antibody per IP as well as the amount of DNA controls to add per IP has been optimized for better specificity. For experiments requiring more than 1 µg, we recommend to perform replicates and then pool the DNA after IP step (as suggested in the manual). If you use more DNA in one IP tube, the amount of the other compounds will have to be titrated, especially the antibody.
