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What is Liquid Chromatography?

April 26, 2017chromatography liquid

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What is Liquid Chromatography?

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Liquid Chromatography is a technique used in laboratories to separate dissolved molecules or ions in liquid phase. The process began in the middle of the 19th century when it was used to primarily separate chlorophyll in plant pigments. Since then this process was developed by Mikhail Semyonovich Tsvet, a Russian botanist using calcium carbonate columns in the early 20th century. There are different kinds of chromatography such as Paper Chromatography, Column Chromatography, Gas Chromatography, Planar Chromatography, Affinity Chromatography, Thin Layer Chromatography and Liquid Chromatography. This article will delve into the details about the latter which is commonly referred to today as High Pressure Liquid Chromatography. There are two subtypes of this process known as the Normal Phase Liquid Chromatography (NPLC) where the stationary phase is more polar than the mobile phase. The other sub-type is the Reverse Phase Liquid Chromatography (RPLC) where the mobile phase is more polar.