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What is known about binding in the presence of nonionic detergents?

April 26, 2017Binding detergents known nonionic presence

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What is known about binding in the presence of nonionic detergents?

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Some fusion proteins do not bind efficiently (< 5% binding) in the presence of 0.2% Triton X–100 or 0.25% Tween 20, while other fusions are unaffected. For one fusion that does not bind in 0.25% Tween 20, diluting the Tween to 0.05% restores about 80% of the binding.