What is known about binding in the presence of non-ionic detergents?
What we know is this: 1) some fusion proteins do not bind efficiently (<5% binding) in the presence of 0.2% Triton X100 or 0.25% Tween 20, while other fusions are unaffected, and 2) for a fusion that does not bind in 0.25% Tween 20, diluting the Tween to 0.05% restores about 80% of the binding. We have reports from researchers that purification in the presence of NP40 works, but because of (1) above we don't know how general this is.
