What is Internal Standard Normalization, and how does it work?
To maximize peak height consistency from instrument to instrument, capillary to capillary and injection to injection, an optional chemistry and software based method of normalization has been introduced in the 3500. If you choose to normalize data, a new version of GS600 LIZ Size Standard (GS600 LIZ Size Standard v2.0) is used and the normalization size standard is specified in the quality control analysis method of the data collection software. During data collection, the software calculates a Normalization Factor for each sample by dividing the Normalization Target (experimentally defined expected peak height) by the observed average peak height of the size standard fragments in the sample. This Normalization Factor is a multiplier and the peak heights of the sample are adjusted accordingly. View the 3500 Series Genetic Analyzers Presentations.
